umuc biol103 Lab 4: Enzymes latest 2015 november

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UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:

·
On your own and without assistance, complete
thisLab
4Answer Sheet electronically
and submit it via theAssignments
Folder by the date listed in theCourse Schedule (underSyllabus).
·
To conduct your laboratory exercises, use the
Laboratory Manual located under Course Content. Read the introduction and the
directions for each exercise/experiment carefully before completing the
exercises/experiments and answering the questions.
·
Save your Lab 4Answer Sheet in the
following format: LastName_Lab4 (e.g.,
Smith_Lab4).
·
You should submit your document as a Word (.doc or .docx) or
Rich Text Format (.rtf) file for best compatibility.

Pre-Lab Questions

How
could you test to see if an enzyme was completely saturated during an
experiment?

List
three conditions that would alter the activity of an enzyme. Be specific
with your explanation.

Take
a look around your house and identify household products that work by
means of an enzyme. Name the products, and indicate how you know they work
with an enzyme.

Experiment
1: Enzymes in Food

Data Tables and Post-Lab Assessment
Table 1: Substance vs. Starch Presence

Table 1: Substance vs. Starch Presence

Substance

Resulting Color

Presence of Starch?

Positive Control: Ginger Root

Negative Control:Student Must Select

Food Product:

Food Product:

Saliva:

Post-Lab Questions
1. What
were your controls for this experiment? What did they demonstrate? Why was
saliva included in this experiment?

2. What
is the function of amylase? What does amylase do to starch?

3. Which
of the foods that you tested contained amylase? Which did not? What
experimental evidence supports your claim?

4. Saliva
does not contain amylase until babies are two months old. How could this affect
an infant’s digestive requirements?

5. There
is another digestive enzyme (other than salivary amylase) that is secreted by
the salivary glands. Research to determine what this enzyme is called. What
substrate does it act on? Where in the body does it become activated, and why?

6. Digestive
enzymes in the gut include proteases, which digest proteins. Why don’t these
enzymes digest the stomach and small intestine, which are partially composed of
protein?

Experiment
2: Effect of Temperature on Enzyme Activity

Data Tables and Post-Lab Assessment
Table 2: Balloon Circumference vs.
Temperature

Table 2: Balloon
Circumference vs. Temperature

Tube

Temperature
(°C)

Uninflated
Balloon Circumference (cm)

Final
Balloon Circumference (cm)

Difference
in Balloon Circumference (cm)

1 – (Cold)

2 – (RT)

3 – (Hot)

Post-Lab Questions
1.
What
reaction is being catalyzed in this experiment?

2.
What is
the enzyme in this experiment? What is the substrate?

3.
What is
the independent variable in this experiment? What is the dependent variable?

4.
How does
the temperature affect enzyme function? Use evidence from your data to support
your answer.

5.
Draw a
graph of balloon diameter vs. temperature. What is the correlation?

6.
Is there
a negative control in this experiment? If yes, identify the control. If no,
suggest how you could revise the experiment to include a negative control.

7.
In
general, how would an increase in substrate alter enzyme activity? Draw a graph
to illustrate this relationship.

8.
Design
an experiment to determine the optimal temperature for enzyme function,
complete with controls. Where would you find the enzymes for this experiment?
What substrate would you use?

 

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