Provide a 7 pages analysis while answering the following question: Molecular Genetics. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required. With the help of a molecular ladder which can be obtained by digestion of the λ DNA with HindIII, the size of the plasmid isolated can be approximated. Lambda DNA on digestion with HindIII breaks into seven fragments of sizes 23.130 kb, 9.416 kb, 6.557 kb, 4.361 kb, 2.322 kb, 2.027 kb, and 0.564 kb. As because the concentration of the digested lambda DNA was less all the digested products could not be visualized i.e. only three bands of the digested DNA which was of larger size could be visualized whereas the smaller fragments could not be visualized. Comparing the band of the plasmid isolated and the bands obtained by the lambda DNA digestion (molecular ladder) it can be approximated that the size of the plasmid isolated is around 3.5-4 kb as the band of the isolated plasmid is further down the third band obtained and would have corresponded to a region between the fourth and fifth bands by digestion of the lambda DNA. The other who ran the gel using the plasmid that they had extracted also estimated the size of the plasmid using the same procedure.


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